9/9/2023 0 Comments Download synapse![]() ![]() Synapses, the key sites of communication in this network, can only be identified and assigned to a specific pair of neurons with electron microscopy (EM). Information processing in the central nervous system is carried out by interconnected neurons. The resource developed here serves as a foundation for synaptic-resolution circuit analyses in the zebrafish nervous system. Moreover, we mapped all 407 presynaptic and postsynaptic partners of two superficial interneurons in the tectum. We reconstructed a network of 208 neurons involved in visual motion processing, most of them located in the pretectum, which had been functionally characterized in the same specimen by two-photon calcium imaging. Neurons and their connections are stored in the form of a queryable and expandable digital address book. We segmented the resulting dataset with the flood-filling network algorithm, automated the detection of chemical synapses and validated the results by comparisons to transmission electron microscopic images and light-microscopic reconstructions. To generate such a resource, we sectioned and imaged a larval zebrafish brain by serial block-face electron microscopy at a voxel size of 14 × 14 × 25 nm 3. Dense reconstruction of synaptic connectivity requires high-resolution electron microscopy images of entire brains and tools to efficiently trace neuronal wires across the volume. ![]()
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